Image Volume Fractionator workflow >
1. Set up the subject
Procedure
Subject Information
Type your name, the subject name, and any notes that you want to include.
Virtual section settings
- Total height of image volume and Distance between image planes are automatically identified.
- Set top (bottom) of ROI: Distance between the top (bottom) of the tissue and the top (bottom) of the virtual section.
To set the top or bottom of the region of interest (ROI), scroll the mouse wheel, or press Page Up/Down on the keyboard to focus through the section then click the SET TOP (BOTTOM) button.
- Number of virtual sections: Number of desired virtual sections you will count for this subject. The appropriate number of virtual sections depends on your experimental goals, the density or sparsity of objects you will count, and the shape and size of your ROIs.
- Virtual section thickness: Automatically calculated virtual section height (the distance between the ROI top and bottom divided by the number of virtual sections).
- Disector height: Represents the height of the volume that you are going to count within the virtual section.