User Guide

Version 2023

About Stereo Investigator software

Stereo Investigator system, which includes both microscope hardware and Stereo Investigator software, is designed to enable you to accurately quantify the number, length, area, and volume of cells, subcellular and macro structures in your tissue specimens, using unbiased stereological methods.

Stereo Investigator makes stereology accessible, with easy-to-follow workflows for the most commonly used probes and clear quantitative results. Using Stereo Investigator, you can perform stereology directly on a microscope system, or from image stacks or whole slide images acquired previously.

• If you are a new user, you may want to tour the software interface here.

What's new

See the What's new page or view the release notes.

Use stereology to further your research

• Acquire representative images using systematic random sampling (SRS image series workflow and SRS image stack series workflow)
• Select a probe based on the type of estimate
• Design a pilot study
• Determine the sampling precision

• Use a workflow, for example:

  • Optical Fractionator workflow for number estimates
  • Spaceballs workflow for fiber-length estimates
  • Connectivity Assay workflow to quantify connections

Resources

Watch a short Stereo Investigator overview video on YouTube (4 min)

• stereology.info: Basic and advanced concepts in stereology for the biological sciences
• Stereo Investigator how-to video playlist on YouTube
• Stereology and Stereo Investigator webinar playlist on YouTube