Enable multichannel acquisition
Purpose
Use this option to acquire fluorescence and false colored images.
Procedure
- Turn on Live image mode.
- The device command sequences necessary for each channel have been defined.
- Configure your system for a multichannel acquisition. See Automating the multichannel acquisition process
- Click Acquire>Acquisition Tool Panels>Show all to open the panels used during the acquisition.
- The Multichannel Control tool panel displays the channels configured in Step 2. .
- Check the Remember Video Settings checkbox.
- Click the device command sequence button for the first channel; adjust the exposure in the camera settings until a full balanced histogram is achieved.
- Click the device command sequence for the next channel; adjust the exposure in the camera settings until a full balanced histogram is achieved.
- Click the device command sequence for the last channel; adjust the exposure in the camera settings until a full balanced histogram is achieved.
The program has "memorized" the camera settings for each channel and is ready to apply them for acquisition.
- Click Enable multichannel in the Capture section of the Acquire ribbon.
- Stereo Investigator performs the acquisition tasks defined for the first channel.
- You may be prompted you to adjust the camera settings. Click OK.
- Stereo Investigator performs the acquisition tasks for the other channels defined.
Optional : Click the Setup button to review the channel's settings.