Neurolucida^® 360 User guide

Version 2024

Overview

Neurolucida 360 software is the premier tool used by neuroscientists to quickly and accurately reconstruct intricate neuronal structures. Create and analyze realistic, quantifiable neuron reconstructions from your microscope images, and perform detailed morphometric analyses.

What's new

See the What's new page or view the Release Notes.

Getting Started

• View a video Introduction to Neurolucida 360 (10:47 min)
• Get an overview of the Neurolucida 360 interface.
• Learn more about working in 3D.
• Complete the one-time set up for using the 3D environment.
• Check out our webinar (1 hr): Online Q/A session—Automatic Neuron Reconstruction and Analysis using Neurolucida 360

Trace & edit in 3D

• Open image files by dragging and dropping them onto the Neurolucida 360 software window. Then specify how you want the program to handle the files in the Image Opener dialog.
• Learn about image scaling
• Make image adjustments before tracing.
• Learn about tracing and editing in the 3D environment.
• Trace trees with the user-guided, automatic, or smart manual tracing mode.
• Model somas, varicosities, spines, and puncta.
• Trace vessels with the user-guided, smart manual, or automatic tracing mode, then edit and classify vessels
• Define regions of interest with contours, and place reference markers.
• Create video clips, and export your 3D representations as vector or image files.
• Use batch tracing in the Batch pipeline to quickly trace over multiple images using a single Neurolucida 360 configuration.

Resources

• Neurolucida 360 How-to Video playlist on YouTube
• Neurolucida 360 webinar playlist on YouTube
• Mouse & keyboard commands / hot keys (PDF)