Select Acquired SRS Image Series

Purpose

 Use the Select Acquired SRS Image Series button to conduct stereology analysis using an image or image-stack series captured using systematic random-sampling (SRS) methodology.

This tool is useful for running the Fractionator and Area Fraction Fractionator probe probes on SRS image series.

Before you start

  1. Capture an SRS image or image-stack series; find instructions for doing that using

    See instructions for acquiring an:

  2. Open the data file for an SRS image or image-stack series using any of the following methods:

    • Drag the data file (i.e., .xml format) onto the Stereo Investigator window.

    • Click the Open data file icon in the quick-access toolbar on the top left of the Main window or in File > Open.

    • Type Ctrl-O on your keyboard and, in the Open window that appears, navigate to and select the data file, then click Open.

Procedure

  1. Go to Probes > Tools and click Select Acquired SRS Image Series.

    The Select an Acquired Systematically Random Samples (SRS) Series for Analysis dialog box opens.

  2. Choose the image series and probe:

    • Select the SRS series from the list.
    • Select a probe from the Run using drop-down list.
    • Check the box to Modify Acquire Settings to enable modification of select SRS-acquisition settings.
    • Click OK.

The stereology probe or probe-workflow selected in the previous step opens.

  • If you chose to Modify Acquire Settings in the previous step, a dialog box similar to that used for the SRS image-series acquisition may open; you can typically change some of the settings (others will be grayed out).
  • Probes that do not have a workflow will typically be at the step where you choose a marker and begin counting.

  • Probe workflows will typically open at the counting step.

See also Fractionator, Area Fraction Fractionator probe, Spaceballs workflow, Optical fractionator workflow