Slide scanning workflow >

8. Sample acquire

Overview

Acquiring a large image can be time consuming; we recommend that you conduct a sample acquire to test the acquisition settings before performing the full slide scan.

This can be helpful to:

  • Evaluate whether scan settings, for example overlap/trim and stage-delay settings are appropriate.

  • Review whether background correction is needed and/or evaluate the result of your background correction settings (5. Scan settings).

  • Preview the effects of Stitching XY and Background subtraction.

Procedure

Check the box to Acquire sample image.

To skip this step, do not check the box and click Next Step to continue the workflow.

Acquire

  1. As per the onscreen instructions, move the stage to bring a portion of the specimen into view.

  2. Choose a scan size using the drop-down menu. 2 x 2 grid is often a sufficient area for the sample.

  3. (optional) Set up focus map...: Click to set up a focus map for the sample acquire. Additional options will be displayed in the window as follows:

  4. Start Scan: Click to start the sample scan. A progress window will be displayed while images are captured.

  5. When scanning is complete, the sample image appears in the Main window and the SlideScan Compile with Preview dialog box opens.