Slide scanning workflow >

7. Background correction

Overview

Background correction minimizes the effects of uneven illumination. It can reduce tiling artifacts in the final compiled image.

Background correction uses a calibrated estimate of the optical field of the microscope to correct each image tile as it is acquired. The calibration is specific to the optical properties of the microscope and is independent of the specimen under study.

In this step, you choose whether or not to include background correction for the slide scan. If included, you will select background correction settings and capture a background correction image.

Procedure

Enable background correction: Check the box to enable background correction for your slide scan.

Depending on the Illumination typeselected (Brightfield or Fluorescent) in step 1. Setup , you'll see different Setup options and instructions:

Instructions for Brightfield microscopy

Instructions for Fluorescence microscopy