4. Set microscope to high magnification

1. On the microscope, select a higher power objective, that will be suitable for viewing objects of interest.
2. Select the corresponding lens from the Select High Mag Lens drop-down menu.

Recommendations

• We recommend using 60x, 63x or 100x oil objectives for counting. Such objectives provide the thin depth of field required to count accurately, especially when cells are on top of each other.

• In general, lower power objectives, such as 10x, 20x, or 40x air objectives, do not have the adequately thin depth of field that is needed to distinguish the unique point of an object, or the top of one cell from another in densely packed regions.