Enable multichannel acquisition

Purpose

Use this option to acquire fluorescence and false colored images.

Procedure

  1. Turn on Live image mode.
  2. The device command sequences necessary for each channel have been defined.
  3. Configure your system for a multichannel acquisition. See Automating the multichannel acquisition process
  4. Click Acquire>Acquisition Tool Panels>Show all to open the panels used during the acquisition.
  5. The Multichannel Control tool panel displays the channels configured in Step 2. .

    6. Optional : Click the Setup button to review the channel's settings.

    1. Check the Remember Video Settings checkbox.
    2. Click the device command sequence button for the first channel; adjust the exposure in the camera settings until a full balanced histogram is achieved.
    3. Click the device command sequence for the next channel; adjust the exposure in the camera settings until a full balanced histogram is achieved.
    4. Click the device command sequence for the last channel; adjust the exposure in the camera settings until a full balanced histogram is achieved.

      The program has "memorized" the camera settings for each channel and is ready to apply them for acquisition.

  6. Click Enable multichannel in the Capture section of the Acquire ribbon.
  7. Neurolucida performs the acquisition tasks defined for the first channel.
  8. You may be prompted you to adjust the camera settings. Click OK.
  9. Neurolucida performs the acquisition tasks for the other channels defined.