SRS Image Series workflow > Acquire Configuration >
6. Counting frame settings
Counting Frame Display
Force the counting frame to be square: Check the box to create a square counting frame as you adjust the frame with your mouse.
Snap to increments of: Check the box and enter the desired rounding increment in µm to round the size of the counting frame to a whole number; this may be easier to remember for future experiments.
Center on live image: If you're working on a live-camera image, you can check the box to center the counting frame in the on-screen image (rather than positioning the counting frame using your mouse).
Counting Frame Size
Size the counting frame to fit approximately 1–5 objects of interest.
- Choose a unique identifying point for your object of interest (e.g., cell) that comes into focus just once, such as:
- Cell top
- Nucleus top
- Nucleolus (unless there are multiple nucleoli in your cells of interest)
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Adjust the counting frame until it is approximately large enough to have, on average, 1 to 5 identifying points that you will count using one of the following methods:
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Type a size (µm) into the boxes labeled X: and Y:.
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Use your mouse to adjust the counting frame size.
We recommend sizing counting frames so that they contain 1–5 objects. This minimizes user error and fatigue; it is difficult to count too many objects per frame, especially in a visually dense image.
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Verify the following:
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The counting frame is roughly in the center of the screen.
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If you're working on a live-camera image, you can check the box to "Center on live image".
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Hover your cursor over the counting frame and drag the mouse to move the frame.
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- There is adequate space outside the counting frame to clearly distinguish objects that are on the edge of the counting frame.
- The tails of the counting frame are visible.
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