Edit Puncta Panel (3D)

To edit puncta and see all the puncta-editing options:

Select the color channel used for puncta detection using either the Channel panel in the 3D environment or the Image Adjustment tool in the 2D window.
Select puncta to be edited.

Selecting puncta

Select puncta for editing using any of the following methods:

Click the Select All button to select all detected puncta.
Hold the Ctrl key on your keyboard and drag your mouse to select all puncta in the marquee (selection box) that forms.
Click a single punctum with your mouse or hold the Ctrl key on your keyboard and click multiple puncta to select.

Selected puncta are displayed with white wireframes.

Remove button: Click the Remove button to delete selected puncta or press the Delete key on your keyboard.
Split button: To separate two puncta that are modeled as one due to physical proximity, select the punctum and click the Split button.
Merge button: To combine two puncta, select the puncta and click the Merge button.

You can merge two puncta at a time. Repeat if necessary to merge more than two puncta into a single punctum.

Show as: Use the drop-down menu to choose how selected trees are displayed.
Color Picker:The color picker to the right of the Show asmenu can be used to change the color used to represent spines. To change the color of selected trees, click the arrow near the colored square and choose a color from the color picker.
Transparency: Drag the Transparency slider to increase the transparency of selected trees.

(You may want to hide trees instead.)
Smooth: Check the box and drag the slider to smooth the appearance of selected trees (they will appear less jagged).

This feature is for visualization purposes only (e.g., snapshots for publications); it does not affect your data.
Surface: Use the drop-down menu to choose a surface effect. (This feature is for visualization purposes only, e.g., snapshots for publications; it doesn't affect your data.)

Click to access additional surface specific controls including gradient colors, transparency, and other context dependent settings.
- Puncta Color: Displays puncta as the color they were originally traced as, or as the color chosen from the Color Picker.
- Distance to Markers: Displays distance (µm) between puncta and markers as a color gradient. Must have a marker present.
- Distance to Spines: Displays distance (µm) between puncta and spines as a color gradient. Must have a spine present.
- Distance to Trees: Displays distance (µm) between puncta and trees as a color gradient. Must have a tree present.