Slide Loader setup 9: Select Virtual Tissue acquisition type
Select the acquisition type for the 3D Virtual Tissue image.
Single channel
Requires a command sequence, typically to set up the light path by moving mirror cubes and filters.
Why?
Running a command sequence before each acquisition enables the persistence of camera settings.
- Before acquire—Requires a command sequence even if the command sequence is "empty" (i.e., it won't lead to a change of state for any device). If there is no existing command sequence, use the Edit Command Sequences button (top right corner of the dialog) to create it.
- After acquire—Optional
Multichannel
Use the parameters already used for the 2D contour image (step 4).
Command sequence before auto-contour acquire
- Select a command sequence from the drop-down menu to maintain a light at a constant setting through the entire 3D Virtual Tissue acquisition.
Some lights require that you add a delay to the command sequence after setting the light level to give the light time to settle at the new level. Using such a command sequence at this step means that the delay only occurs once instead of occurring before each tile.
Adjust Virtual Tissue camera settings
See Slide Loader Virtual Tissue Setup Workflow
Stereo Investigator 11 | MBF Bioscience Support Center | Downloads