Slide Loader setup 4: Select auto-contour acquisition type
Select the acquisition type for the 2D Virtual Tissue image.
Acquisition Type
Brightfield
This type may be more suitable to obtain a good contour, even if you plan on acquiring a multichannel fluorescent 3D Virtual Tissue.
Single channel
Requires a command sequence, typically to set up the light path by moving mirror cubes and filters.
Why?
Running a command sequence before each acquisition enables the persistence of camera settings.
- Before acquire—Requires a command sequence even if the command sequence is "empty" (i.e., it won't lead to a change of state for any device). If there is no existing command sequence, use the Edit Command Sequences button (top right corner of the dialog) to create it.
- After acquire—Optional
Multichannel
To use this type, define command sequences with the Multichannel Acquire Setup button. See Acquire Setup: Automating tasks for the acquisition of multichannel images / Multichannel Acquires tab for details.
Use the same parameters for the 3D Virtual Tissue multichannel acquisition (step 9).
Multichannel settings do not persist.
Command sequence before auto-contour acquire
- Select a command sequence from the drop-down menu to maintain a light at a constant setting through the entire 2D Virtual Tissue (auto-contour) acquisition.
Some lights require that you add a delay to the command sequence after setting the light level to give the light time to settle at the new level. Using such a command sequence at this step means that the delay only occurs once instead of occurring before each tile.
Adjust auto-contour camera settings
See Slide Loader Virtual Tissue Setup Workflow
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