Select the acquisition type for the 2D Virtual Tissue image.
Brightfield
This type may be more suitable to obtain a good contour, even if you plan on acquiring a multichannel fluorescent 3D Virtual Tissue.
Single channel
Requires a command sequence, typically to set up the light path by moving mirror cubes and filters.
Multichannel
To use this type, define command sequences with the Multichannel Acquire Setup button. See Acquire Setup: Automating tasks for the acquisition of multichannel images / Multichannel Acquires tab for details.
Use the same parameters for the 3D Virtual Tissue multichannel acquisition (step 9).
Multichannel settings do not persist.
Some lights require that you add a delay to the command sequence after setting the light level to give the light time to settle at the new level. Using such a command sequence at this step means that the delay only occurs once instead of occurring before each tile.
Adjust auto-contour camera settings
See Slide Loader Virtual Tissue Setup Workflow
Stereo Investigator 11 | MBF Bioscience Support Center | Downloads