Creating a new lens or calibrating an existing lens from an image

Purpose

For each objective in use, you need a corresponding software lens. Use this procedure to create software lenses from images with known scaling or that contain an accurate scale bar.

Software lenses can also be used to assign scaling information to image files that:

  • Do not contain scaling information.
  • Contain inaccurate (wrong) scaling information.
  • Contain scaling information that Stereo Investigator cannot recognize/interpret.

Materials needed

An image with known scaling, for example one of the following:

  • Image file that includes scaling information, for example, such as .jp2 or .jpx files captured using an MBF Bioscience imaging system

  • Image of the MBF Bioscience calibration-grid slide or a graticule slide with known scaling:

    Acquire an image of the calibration-grid slide using the same objective that you plan to use in your study.

    If you are using multiple objectives, acquire an image of the calibration grid slide with each objective.

    • 10x and lower: Acquire an image of the large grid (250 µm).
    • 20x and above: Acquire an image of the small grid (25 µm). Immersion media (oil, water, etc.) are not required for this step.

  • Image that includes an accurate scale bar

Procedure

Using an image file that includes scaling information to create a new lens

Using an image of a calibration-grid slide or an image that includes an accurate scale bar to create a new lens

Also see Calibrating your System