This step enables you to set up a test region for the settings used in Step 3 - Detect Spines.
Use this option when working with a dense, complex image. This enables you to test your parameters on a small region that you consider typical of the branches under investigation relatively quickly.
Use this option when working with a dense, complex image. This enables you to test your parameters on a branch that you consider typical of the branches under investigation relatively quickly.
Use this option to detect spines on the entire tracing.