Enable Illumination Correction

Turn on Enable Illumination Correction before acquisition if you are unable to use a background correction with an actual background image.

Note that:

• Illumination correction is designed for fluorescent images -- applying this command to other types of images is likely to yield poor results.
• This command is CPU-intensive.
  
• It may noticeably increase the exposure of the sample to light. We recommend processing images with easily bleachable dyes after acquisition as well as 3D single-plane acquisition.
  

Before applying to a virtual tissue, we recommend that you experiment with different settings on a test tissue.

 

Using Illumination Correction

1. Before acquiring an image, click Acquisition>Enable Illumination Correction. Neurolucida displays the Illumination Correction dialog box .
   • Type: Start with Gentle.
   • Light Removal: Start with 50%.
   • Target Channel: Disabled (available for acquired images). Image Illumination Correction
2. Click OK and perform the acquisition.
3. Examine the acquired image. If you are not satisfied with the results:
   1. Click Acquisition>Enable Illumination Correction again. Neurolucida disables the command.
   2. Click Image>Live Image to return to the live image.
   3. Click Acquisition>Enable Illumination Correction again to select different settings.
   4. Perform the acquisition.

How it works

In the absence of a background image, Neurolucida uses the image to estimate illumination variance for background correction.

 

Also see Enable Background Correction, or Image Illumination Correction for acquired images.

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