All very large images (larger than 2500 by 2500 pixels) are loaded in virtual image mode in Microlucida.
In the Image Organizer, Microlucida specifies "(Virtual Image Mode)" in the image details.
Messages may be displayed indicating that you that you are in virtual image mode.
In virtual image mode, Microlucida only loads the area of the image that you see displayed in the Tracing window.
When you navigate to a different area in the image or when you zoom in or out, Microlucida loads the corresponding area and displays it in the Tracing window.
Here are some of the implications:
The .pff format is loaded in standard mode (i.e., not in virtual image mode) unless you specify that you want to use the virtual image mode whenever possible.
Yes, you can if you do the following:
You can save a single image from a stack (current plane) or projection images at full resolution.
The "virtual image mode" and "virtual tissue" are two unrelated concepts.