Slide Loader setup workflow >

9. Select acquisition type

Select the scanning type for the 3D slide.

 

Single channel

Requires a command sequence, typically to set up the light path by moving mirror cubes and filters.

Running a command sequence before each acquisition enables the persistence of camera settings.

  • Before acquire—Requires a command sequence even if the command sequence is "empty" (i.e., it won't lead to a change of state for any device). If there is no existing command sequence, use the Edit Command Sequences button (top right corner of the dialog) to create it.
  • After acquire—Optional

Multichannel

Use the parameters already used for the 2D contour image (step 4).

 

Command sequence before auto-contour acquire

Select a command sequence from the drop-down menu to maintain a light at a constant setting through the entire 3D slide scan.

Some lights require that you add a delay to the command sequence after setting the light level to give the light time to settle at the new level. Using such a command sequence at this step means that the delay only occurs once instead of occurring before each tile.