Rapid Multi Region Scanner
The Rapid Multi Region Scanner for fast and flexible 2 photon imaging. Powered by ScanImage®Product Overview
The Rapid Multi Region (RMR) scanner increases your microscope’s imaging rate by subdividing the field of view into multiple regions of interest. Its novel design combines the flexibility of two galvos and the speed of a resonant scanner into an integrated, compact device. The scanner is fully compatible with ScanImage‘s powerful scanning modes. The flexible mounting options allow straightforward installation on a number of commercial 2P microscopes, including the Thorlabs BScope and Sutter MOM, as well as DIY microscopes.
Key Benefits
- Increases your microscope’s imaging rate
- Allows scanning of multiple regions of interest - so no time is wasted in empty areas of the field of view
- Performs arbitrary line scanning - to sample individual neurons along a predefined scan path for maximum speed
- Performs photostimulation - to rapidly stimulate neurons in sequence
The RMR is a resonant-galvo galvo module for 2P microscopes. It was developed as a novel solution for neuroscientists seeking to scan multiple regions of interest with high time resolution.
The RMR is superior to typical resonant-galvo configurations, in which if you have several small regions-of-interest (ROIs) within a larger field-of-view, you could scan the full field of view and then only analyze the ROIs you are interested in. However, you pay a price in frame rate because you’re spending a lot of time raster scanning areas that you’re not interested in.
The additional galvo in the RMR allows you to raster scan a small region and then move that around in X and Y.
| Integrated resonant and 2x galvo mirrors |
| Compatible with Thorlabs Bscope II, Sutter MOM, and DIY microscopes |
| 2x Cambridge galvo 6215H mirrors |
| Cambridge resonant mirror 8kHz |
| Group delay dispersion < 1000fs^2 |
| FOV: 20 degree optical |
| Suitable for a laser beam diameter up to 4mm |
| 30mm cage mountable |
| Controller box included |
| Wavelength range: 800nm - 1100nm |
| Description | Value | Unit |
| Input Voltage Nominal | 100-120 | V (AC) |
| Input Voltage Range | 90-132 | V (AC) |
| Input Voltage Frequency | 50-60 | Hz |
| Power Consumption (maximum) | 1050 | W |
| Pollution Degree | IPX0 | (unprotected) |
| Input Beam Diameter | < 4 | mm |
| Group Dispersion Delay | < 1000 | fs2 |
| Maximum Galvo Line Rate at Full Amplitude | < 1000 | Hz |
| Resonant Scanner Frequency (Nominal) | 8000 | Hz |
| Galvo Scan Angle | +-10 | degrees (optical) |
| Resonant Scan Angle | +-13 | degrees (optical) |
| Usable Resonant Scan Angle Before Clipping Occurs | +-10 | degrees (optical) |
| Weight Scan Head | 1.5 | kg |
| Dimensions Scan Head | 180 x 202 x 50.8 | mm |
| Dimensions Controller Box | 483 x 364 x 89 | mm |
| Weight Controller | 7 | kg |
| Maximum Noise Level at 1m distance Lpl(20 µPa) | 45 | dB(A) |
Lu, J., Behbahani, A.H., Hamburg, L. et al. Transforming representations of movement from body- to world-centric space. Nature 601, 98–104 (2022). https://doi.org/10.1038/s41586-021-04191-x
Download Rapid Multi Region Scanner product sheet here.
RMR Scanner: Key Features
Rapid multiple region scanning
Combine the speed of resonant raster scanning with the flexibility of galvo scanning. By selecting regions of interest, no time is wasted in empty areas of the field of view. ScanImage’s intuitive mROI feature makes it easy to define regions in 2D or 3D.
RMR Scanner: Key Features
Arbitrary line scanning
Use the galvo pair to sample individual neurons along a predefined scan path. ScanImage’s powerful arbitrary line scanning feature allows to quickly define 3 dimensional paths and automatically optimizes the transition between features for maximum speed.
RMR Scanner: Key Features
Photostimulation
Use either a resonant raster scan and selectively increase the power in selected regions using ScanImage’s Power Box Feature, or use the Photostimulation Feature to rapidly stimulate neurons in sequence.
Who Is Using RMR Scanners?
RMR scanners are used across the globe by the most prestigious laboratories.
Cited in Peer Reviewed Scientific Publications
MBF’s software utility is underscored by the number of references it receives in the worlds most important scientific publications. See examples below:
Qin, Y., X.-Y. Zhang, et al.
Downregulation of mGluR1-mediated signaling underlying autistic-like core symptoms in Shank1 P1812L-knock-in miceView Publication
Rentsch, P., T. Egan, et al.
The ratio of M1 to M2 microglia in the striatum determines the severity of L-Dopa-induced dyskinesiasView Publication
Zhang, X., C. Wang, et al.
Analysis of Error Sources in the Lissajous Scanning Trajectory Based on Two-Dimensional MEMS MirrorsView Publication
Jiang, Y., M. Huang, et al.
Full-Length Transcriptome Analysis of Soybean Cyst Nematode (Heterodera glycines) Reveals an Association of Behaviors in Response to Attractive pH and Salt Solutions with Activation of Transmembrane Receptors, Ion Channels, and Ca2+ TransportersView Publication
Lu, J., Behbahani, A.H., Hamburg, L. et al.
"Transforming representations of movement from body- to world-centric space."
Paletzki, R. and C. R. Gerfen
“Whole Mouse Brain Image Reconstruction from Serial Coronal Sections Using FIJI (ImageJ).”View Publication
Leung, C., S. Robbins, et al. (2021).
"3D single cell scale anatomical map of sex-dependent variability of the rat intrinsic cardiac nervous system." iScience 24(7): 102795.View Publication
Kim, S.-H., S. H. Hadley, et al.
"Mapping of Sensory Nerve Subsets within the Vagal Ganglia and the Brainstem Using Reporter Mice for Pirt, TRPV1, 5-HT3, and Tac1 Expression." eNeuro 7(2): ENEURO.0494-0419.2020.View Publication
Frequently Asked Questions (FAQ)
Can I use the RMR scanner in a galvo-galvo configuration?
Yes. It is not required to drive the resonant scanners all the time. You can use it only for experiments where it is needed and leave it disabled for others.
Does the RMR scanner require ScanImage to operate?
No. The RMR scanner can be operated by any system that can generate the appropriate control signals.
I need to perform resonant scanning at 12kHz. Is this possible?
We can custom-build an RMR scanner with a 12kHz resonant scanner. Please contact to discuss this option.
What microscopes are the RMR compatible with?
The RMR has flexible mounting options allows installation on a Thorlabs BScope, a Sutter MOM and DIY microscopes.
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Robust Professional Support
Our service sets us apart, with a team that includes Ph.D. neuroscientists, experts in microscopy, stereology, neuron reconstruction, and image processing. We’ve also developed a host of additional support services, including:
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