Label analysis

Labels are used to define areas of interest in XY and time intervals of interest to refine tracking and/or analyses.

You can find label-specific results for:

Speed/Moving average speed/Smoothed speed: refers to the distance per second covered by the worm along its central axis.

Length/Width/Area/Wavelength: The Length/width/area/wavelength analyses are applied to labels.

Fit: Quantifies how well the worm model conforms to the underlying image data. The higher the number, the better the fit. The value 1.0 represents a perfect fit.

Worm density: Refers to the minimum, maximum, or mean number of worms per frame within a label. To apply changes to the Display type (histogram or x,y plot) or the Operation (mean, min, or max), click Refresh Data.

Track count: Refers to the number of worm tracks within a label.

Label overlap: Reports the time points when worm head/midpoint/tail (user selected) enters and exits a label region.

Track summary by label: Provides all metrics from the Track Summary report for a given label.

Note that there are two ways to use labels in the time dimension: 

  • Use a label once between frame x and frame y.
  • Use a label multiple times (this is a recurring label): Define the starting frame, the time length and the number of frames before the next starting frame to repeat the label.

Viewing results for recurring labels

There are two options to present your data: Aggregate results (calculated over the entire video) OR split results (results specific to each period of time corresponding to the recurring label).

To view split results, check "Split recurring labels" and click "Refresh data" in the Parameters panel.

Example

You defined this recurring label:

You are interested in the moving average speed.

  • The aggregate result shows the moving speed averaged for the entire video.
  • The split results show the moving speed averaged for each period of time corresponding to the recurring label.

Graphical display

Use the Parameters panel to change the graphical representation.

You can show histograms or plots for mean, minimum, or maximum values. If you check "Split recurring labels," you will see results specific to each period of time corresponding to the recurring label.

 

See About labels, Label editor (creating labels), Analyzing data using labels