WormLab User Guide

Version 2024

Take a tour of WormLab software.

What's new

View the current Release Notes PDF or see all Release Notes.

New floating license option is now available for the Mac version. This feature increases flexibility enabling your WormLab license to run on different computers. Please note: requires current software upgrade and support subscription, and internet access. Click here to renew your subscription.

New option in Video playback controls to help optimize performance of videos with variable keyframe intervals. This can enable faster/smoother scrolling through videos.

Batch Processing Enhancements
- Videos may be directly added to the tracking queue.
- A previously saved configuration can apply the same tracking parameters to multiple videos in the tracking queue.
Expanded the Configuration Manager to save/load labels in addition to tracking parameters.
Added Apply button to test Head/Tail detection settings. This enables you to immediately see the results of head/tail detection without having to complete tracking.

Added Integral Measurement (Full/Positive/Negative) to the Report tab of Bending Angles – Multiple analysis. This can be used to provide insights on the symmetry of the bending behavior of the worm.
New Label Overlap analysis reports time point when worm (head, midpoint, tail – user selectable) enter/exits a label region. This is useful for monitoring dwell time within a region of interest.
Enabled multiple projects selection and deletion in Group analysis.
Added Number of Omega Bends and Omega Bend Time to Track Summary analysis.

New floating license option available that increases your flexibility by enabling you to run WormLab on different computers with a single license. Please note: Floating licenses require a current software upgrade and support subscription and internet access. Click here to renew your subscription.

Back tracking is now optimized significantly, improving throughput.
Head/tail assignment utilizes predominant movement direction for low resolution videos
Default values for overlap/interaction tracking are now based on video frame rate
Enhanced Multiple Hypotheses Tracking algorithm for improved tracking through entanglements, coiling and self-overlap.

Added Export capability for a consolidated Bending Angles report
Enhanced label analysis and track summary
You can now define the active region of the label to be inside or outside the specified region of interest.
All metrics previously available in Track Summary are now available in Track Summary by Label

New, more efficient video encoder improves encoding speed and decreases file size
Increased available buffer to support fast frame rate recording using high resolution cameras
Added support for latest Basler cameras. Pylon version 6.2.0 or later is required.

Peristaltic distance and speed metrics better describe worms predominantly moving in reverse
Pirouette analysis has been added
The track summary now includes cumulative mobility forward, idle, and reversal times
Improved handling of large datasets

Added tools to easily remove non-worm objects from data sets
New ability to assign a specific ID to a worm track
Automatically join discontinuous worm tracks based on user-defined shape and position tolerances
Easily audit tracking results with navigation tools to quickly jump to key events in a worm track (e.g. track start, termination, entanglements or self-overlap)
Worm re-indexing is now reflected in the Repair tab

Learn about repair tools.

Improved support for videos with variable keyframe intervals enables faster/smoother scrolling through videos
Free-hand contour label option enables flexible delineation of user-defined regions of interest
Added support for macOS Big Sur
Added option to specify a fixed com port for systems where auto scanning ports may fail
Measuring tool for scaling may be drawn at any angle
WormLab Explorer, an analysis-only licensing option is now available (contact sales@mbfbioscience.com for details)