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Slide scanning workflow

 

Available from the Acquire ribbon bar.

Use this workflow to scan at any magnification and create high resolution 2D images or 3D stacks. Here is how it works: First, a series of individual image tiles are scanned using a motorized stage. The tiles are then merged to form a single image montage called a virtual slide.

Before you start, make sure that you have a properly calibrated system to obtain high-quality images, then follow the workflow's steps. The workflow will vary depending on the options you select in step 1. You will be directed to the steps that are relevant to your selections.

 

Image scaling is not embedded in the .tif files generated via slide scanning.

 

ClosedSee the workflow steps
  1. Set up
  2. Set tracing magnification
  3. Trace
  4. Set acquire magnification
  5. Region selection
  6. Background correction
  7. Scan settings
  8. Sample acquire
  9. Define focus map
  10. Start scanning

 

You also have the option to scan slides without the workflow. Carefully follow these instructions if you choose to do so.