About markers

A variety of marker types (i.e., circle shape, star shape, etc.) are available in the Markers toolbar in 2D.

Placing markers

1. Click a marker type in the Markers toolbar.
2. Click in the Tracing window to place markers over the neuronal structures.

While you are in Markers mode, the tracing functionality is disabled. To return to tracing, click the marker type that is highlighted in the Markers toolbar; this will deselect the marker type and turn off the Markers mode.

Deleting markers

To delete the last marker placed, press CTRL-Z

To delete all markers of the same marker type, right-click over the marker type in the Markers toolbar and select Delete All Markers of This Type.

Editing markers

While there is a comprehensive menu of editing options in the standard window (2D), note that there are also several editing options available in the 3D visualization window.

See Editing markers (2D) and Editing markers (3D)

Using markers for colocalization

Colocalization markers combine 2, 3, or 4 markers into one marker. Use these markers to label cells and structures that were prepared with two or more staining methods. See Colocalize markers for details.

Customizing the Markers toolbar