Outline sections

Purpose

Outline tissue sections in an image automatically. The tool creates contours to outline sections that are visually distinct from the background.

Procedure

Before you start

Open an image; note that Stereo Investigator Whole Slide Edition supports analysis of 2D or single-plane images only.

Outlining sections

  1. Go to the Pipeline ribbon and click Outline sections.

    Stereo Investigator Whole Slide Edition automatically detects each tissue section in the image and generates contours that outline the sections.

  2. If needed, you can edit the contours to more accurately outline the sections using contour editing tools.

    When sections are spaced closely, more than one section may be outlined with a single contour. To split the contour:

    1. Right click a point near the space between the sections and choose Split Contour at this Point.

    2. Repeat at the other side of the contour. You will now have two "open" contours.

    3. Right click one of the open contours and select Close selected contour. Repeat for the other open contour.

    4. If needed, reshape the straight line that was drawn to close the contours.

  3. (optional) Add sections to the Serial Section Manager by clicking New Section. All of the contours will be added to the Serial Section Manager.

See also Serial Section Manager, Selecting and editing contours (2D)