Cavalieri Estimator - Marking Modes

To mark a point, click in the vicinity of a grid point(+): your marker is placed exactly on the center of the grid point .

To disable Snap to Grid:

1. Select the Options>Stereology Preferences>Cavalieri tab.
2. Uncheck Allow Snap to Grid for Cavalieri Probe and click OK.

To place markers in clusters (rather than one at a time), right-click and select Paint Cavalieri Markers Mode or Marquee Mode.

The cursor looks like this .

To mark a cluster of grid points, drag the mouse over the area of interest.

To adjust the size of the circular cursor, use the "+" and "-" keys of the numerical keypad on your keyboard OR use the mouse wheel.

• To delete a cluster of markers, drag the mouse over the area of interest.
• To delete markers within the diameter of the cursor, click near the markers.

To place a cluster of markers within a rectangular area:

1. Drag from top left to bottom right.
2. Release the mouse button.

To delete a cluster of markers within a rectangular area:

1. Drag from bottom right to top left.
2. Release the mouse button.

To fill a contour with markers:

1. A contour is already drawn.
2. Right-click and select Paint Cavalieri Markers Mode.
3. Right-click again and select Paint Markers into Contours.

To remove all the markers from a contour, right-click again and select Erase Markers into Contours.

The contour must be accurately traced to the boundaries of the region of interest. If not, manually add or remove markers around the edges of the contour.