Save/ Save as image stack
May require a separate license
Purpose
Save a multiplane image—an image that include multiple Z-planes |
Procedure and options for saving your image stack
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Go to File >
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Save As if you're saving a file for the first time or want to save a file with an updated name or location. This will not change the original file.
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Save if you want to save updates to a file that has been previously saved and you do not want to update the file name or location. This will overwrite the original file.
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Click the Image Stack button.
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From File > Save, clicking Image Stack saves the file and returns you to the previous Stereo Investigator view.
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From File > Save As, clicking Image Stack opens the Image Stack Save As window. Choose options and save or Export as described in the next step.
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In the Image Stack Save As window, choose options and save or export the image stack as follows:
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Navigate to the desired location for the file and type in a File Name.
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(optional) Choose from the following options:
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Save/Export as type drop-down menu:
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MBF JPEG2000 is the default file type: we recommend it.
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MBF JPEG2000 and MBF TIFF files can include high bit-depth pixel data (if present) and image-display adjustments you've made in Stereo Investigator. Metadata about the image, such as the microscope hardware used, is also saved.
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JPEG2000 file format offers smart compression that can result in a significant reduction in the amount of storage space needed, with minimal data loss.
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TIFF file format is uncompressed.
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Export the image file: Check the box to save (export) the image only and break its link to any associated data file.
When checked, the Save button changes to an Export button.
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The original image-display information and any high bit depth data that were present are lost.
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This enables selection of additional Image Detail [or Stack Save Options] options.
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Stack Save Options:
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Save as individual channels [available for multiple color-channel images]: Saves each color-channel in the image as a separate file.
The channel number is added to the end of the file name, e.g., "FileName_Ch01.jp2"
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Save image color [available when you select Save as individual channels]: Saves the color information with each separate channel or stack.
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Save as a series of single image files: Save each image plane as a separate file.
The plane is added to the end of the file name, e.g., "FileName_001.jp2" or "FileName_001_Ch01.jp2"
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Compression [available when either JPEG2000 file type is selected]: Use the slider to indicate the amount of file compression desired.
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Click the Save or Export button to save/export the file and close the dialog box.
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