Editing a lens
See Creating a Lens to add a software lens.
Purpose
Use Edit Lens to edit and delete existing software lenses.
Procedure
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Go to File > Calibration and click Edit lens.
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In the Select Lens for Editing window that opens:
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Select the lens you want to edit or delete from the list.
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Click the Edit button.
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In the Edit Lens Parameters window that opens you can delete or edit the lens:
Deleting a lens
Click the Delete Lens button.
Editing a lens
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Change the basic lens information or parameters as needed.
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Click OK to save your changes.
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Lens Parameters
The following lens parameters are displayed in the Edit Lens Parameters window and can be edited:
Basic lens information
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Name: Names should provide unambiguous identification of the magnification and the viewing mode (video camera or Lucivid).
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Comments: (optional) Comments can help you identify the lens and its conditions of use; they are stored with the lens calibration file (.len filename extension).
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Units: the unit should be appropriate for the lens, typically micrometers.
Scaling Information
You can enter Scale Factor and/or Aspect Ratio in the corresponding field.
Display resolution is also shown, but is not editable
Lens Category
The lens category affects the calibration parameters, so it's important to set category appropriately:
- Camera or Image: lenses used with previously acquired images or with a camera
- Lucivid: Direct viewing through the oculars of the microscope
Z Correction factor
Radio buttons that correspond to common lens media auto-populate the Z correction factor. Alternatively, you can select User-Specified and type in the refractive index of the medium between the lens and the sample.
Light refraction between the microscope lens and the specimen may cause a difference between the physical movement of the microscope hardware and the position of the focal plane. The Z correction factor is used to calculate the true position of the focal plane as the distance between the microscope lens and the top of the specimen changes.
Typically, the predefined values for air, oil, etc. immersion lenses can be used for Z correction. There are circumstances, however, in which the predefined settings should not be used. Contact MBF Bioscience Technical Services for information about unusual combinations of lens and mounting medium.