Neurons in Serial Sections—2: Trace in the First Section
Regions of interest/fiducial contours have been traced.
Trace the neuronal processes in the first section:
- Switch to an objective that allows you a clear view of the neuronal processes to be traced.
- Focus on the top or bottom of the section; the Z meter value should correspond to the Z value in the Serial Section Manager.
- To display the Z meter, click the icon
. - To change the value, use Move>Set Current Z.
- To display the Z meter, click the icon
- Click Trace>Manual Neuron Tracing and trace all the processes in the section.
- See Tracing Trees in Single Sections (2D) for detailed instructions on tracing processes.
- When placing endings, use the Z meter to determine if the endings are high (i.e., at the top of current the section) or low (i.e., at the bottom of the current section).
Move to the subsequent section and align tissue and tracing from the first section 