Closed Surface analysis

Vessel reconstruction and analysis is available with the Neurolucida 360 Ultra package only. Click here for more information.

Purpose

Use Closed Surface analysis to measure or count structures or portions of structures within a 3D structure generated by adding a skin to closed contours that are aligned in the Z direction.

Procedure

1. Select the structures you want to analyze.

2. Go toAnalyze>Anatomical> and clickclosed surface Analysis.

   1. In theClosed Surface Analysesdialog that opens, check the boxes to select the analyses you want to run

   2. ClickOKto generate the results.

For puncta only, you can alternatively go toAnalyze>and clickPunctum, then select Puncta in closed surfaceand clickOK.

In the example below, an axon enters and exits the 3D structure four times. In the "length" column, you can see the length of each part of the axon that is contained within the 3D structure.