Closed Surface analysis

Vessel reconstruction and analysis is available with the Neurolucida 360 Ultra package only. Click here for more information.

Purpose

Use Closed Surface analysis to measure or count structures or portions of structures within a 3D structure generated by adding a skin to closed contours that are aligned in the Z direction.

Procedure

1. Select the structures you want to analyze.

2. Click to access closed surface analysis for neuronal trees and vessels, or puncta as follows:

   • Axons, dendrites, and vessels: Select Closed Surface Analysis from the Anatomical drop-down menu on the Analyze ribbon.

   • Puncta: Click Punctum on the Analyze ribbon and select Puncta in closed surface. Click OK.

In the example below, an axon enters and exits the 3D structure four times. In the "length" column, you can see the length of each part of the axon that is contained within the 3D structure.