Segment (branched structure)

Use Segment analysis to obtain the finest details for branched structure analysis. A segment is defined as any portion of a branched structure with endings that are either nodes or terminations with no intermediate nodes.

Analysis results

Tree: Each branched structure (tree) selected is assigned a unique number beginning with 1.

Order: Centrifugal branch order of the segment. About branch orders

Length (µm): Total length of the line segments used to trace the segment.

Tortuosity: Tortuosity = [Actual length of the segment] / [Distance between the endpoints of the segment]

  • The smallest value is 1; this represents a straight segment.
  • Tortuosity increases as the segment assumes a more complex path to reach its destination.
  • Tortuosity allows segments of different lengths to be compared in terms of the complexity of the paths they take.

Surface Area (µm²)/ Volume (µm³): Computed by modeling the pieces of the segments as frusta (i.e., truncated right circular cones).

Terminal Type: Describes the endpoint of the segment that is distal to the origin.

Planar Angle (°): Computed based on the endpoints of the segments. It refers to the change in direction of a segment relative to the previous segment. Consequently, the first segment in a tree does not have a planar angle and is marked N/A.

XY Angle / Z Angle (°): XY and Z angles form the spherical coordinate angles for the end of the first segment relative to the start of the next segment.

Maximum Angle (°): Defined only for segments that end at nodes. The value is the maximum of the planar angles of the daughter segments (i.e., the other segments that are attached to the node).

Base Diameter (µm): Diameter at the start of the segment.

Average Diameter (µm): Length-weighted mean. The segment is modeled as a series of frusta. The length of each frustum is used in the calculations to provide a correct average diameter over the length of the segment as it tapers.

Base Coordinates: The base coordinate is the (X, Y, Z) position of the start of the segment.

If you selected additional branch orders, the data is displayed between the Order and the Length columns.

  • To select additional branch ordering methods, go to Preferences in the File menu and select Ordering and select the desired branch orderings from the Analysis Branch Orderings list.
  • To obtain shaft data, assign Shaft ordering to your tracing while working in Neurolucida or Neurolucida 360 (see Branch Ordering in the Neurolucida or Neurolucida 360 user-guide).