Layer length (branched structure analysis)

Purpose

Layer Length analysis is used in conjunction with open delineations created in Neurolucida or Neurolucida 360 software to quantify branch structures within anatomical or other regions of interest.

Procedure

1. In Neurolucida or Neurolucida 360: Create and define the layers by drawing open contours and defining them. Save the data file.
2. In Neurolucida Explorer: Open the data file.
3. Select the Contour and Dendrite or Axon labels in the Traced structures panel.
4. Select Branch Structure Analysis from the Structure drop-down menu on the Analyze ribbon, and select Layer Length.

Analysis results

Layer: Layer names are the names of the open delineations defined in Neurolucida or Neurolucida 360.

Length: Total length of process contained in the layer.

Nodes: Number of nodes contained in the layer.

Spines: Number of spines contained in the layer.