Step 3: Preview
Detection
Channel: Select the appropriate channel. If you select Adjusted display image under image source, the channel is automatically set to Adj. image. 
Preset: Select a preset to apply the same parameters to multiple images. If there are no presets, you'll have the opportunity to save the parameters for this detection when you finalize the detection (Step 4).
About presets
Saving or deleting presets
- Click Presets
and select Edit Presets. - In the Save/Update window:
- Type a name for the preset, and then click Save.
OR
- Select an existing preset and click Delete.
Image type
Select the correct type.
Image source
Raw data: No changes to how the image appears.
Adjusted display image: Modify the Histogram in Image Adjustment to optimize the display for detection. If you select this option, the channel is automatically set to Adj. image.
Color selection
Select a color that contrasts well with the image data to be used for the detection markers and for the centers of the cells that are being detected.
Preview parameters
Set an approximate size for the largest and smallest cells to be detected (enter a value or use the Measure buttons) then click Preview.
Run preview detection on displayed image area: Detection is restricted to the portion of the image displayed in the tracing window for a quicker preview.
Strength
Drag the slider for an instant preview.
The higher the strength, the more background noise is removed.