Create a new lens/Edit a lens: Parameters

Each objective on the microscope has different calibration parameters depending on the viewing mode (video camera or Lucivid) and the magnification.

• Choose a name that permits unambiguous identification for each viewing mode.

Add comments to help you identify the lens and its conditions of use. These comments are inserted into the lens calibration file (‘stereo.len’) and do not appear elsewhere.

The comments are only displayed when editing a lens.

Change to millimeters or nanometers for data tablet use or EM.

• Lucivid: Direct viewing through the oculars of the microscope
• Camera or Image: Video lenses are used with a camera.

Displays the factor detected in the file. If the value is incorrect, enter the correct value manually.

Displays the ratio detected in the file. If the value is incorrect, enter the correct value manually.

This factor is used to calculate the true position of the focal plane as the microscope is moved up and down.

The focal plane moves a distance that may differ from the movement of the microscope. The difference is due to the refraction of light between the cover slip and the immersion medium of the lens. So the factor is usually the inverse of the index of refraction between the tissue and the lens immersion medium.

Under normal circumstances the predefined values for air, oil, and water immersion lenses can be used. But there are circumstances in which the predefined settings should not be used; contact us for information about unusual combinations of lenses and mounting media.

• Select User-Specified to enter your own correction factor.

Box Size

• Use 250 µm (large grid) for lower than 10x objectives.
• Use 25 µm (small grid) for 10x and above objectives.

Force Square

Use with acquired images where a calibration square is not available and calibration must be performed with a calibration line or bar.