Deep Focus

Use Deep Focus to visualize an entire image stack in a single plane. To do this, BrainMaker software collapses the focus information from the 3D image onto a single image plane; it creates a new image and places it at the Z-depth of the top of the stack.

• To switch between the projection view and the stack, use the Image Organizer .
• To save the Deep Focus image, use File>Save>Virtual Image Stack>Deep Focus.

How it works

Deep focus identifies the pixels with the highest sharpness value, whereas minimum/maximum projection identifies the maximum (or the minimum) intensity value of each pixel along the z axis is projected onto the 2D plane.

See Maximum / Minimum intensity projection for a similar, alternative method.