Channel: Select the appropriate channel. If you select Adjusted display image under image source, the channel is automatically set to Adj. image.
Preset: Select a preset to apply the same parameters to multiple images. If there are no presets, you'll have the opportunity to save the parameters for this detection when you finalize the detection (Step 4).
OR
Markers/preview: Select a color that contrasts well with the image data to be used for the detection markers and for the centers of the cells that are being detected.
Select the correct modality.
Original image: No changes to how the image appears.
image Adjustment result: Modify the Histogram in Image Adjustment to optimize the display for detection. If you select this option, the channel is automatically set to Adj. image.
Set an approximate diameter for the largest and smallest cells to be detected (enter a value or use the Measure buttons) then click Preview.
Run preview detection on displayed image area: Detection is restricted to the portion of the image displayed in the tracing window for a quicker preview.
Filtering Slider: Drag the slider for an instantaneous preview. The higher the value, the more background noise is removed.