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Image Illumination Correction

Use Image>Modify image>Illumination Correction when working with images already acquired.

Illumination correction is designed for fluorescent images -- applying this command to other types of images is likely to yield poor results. This command is CPU-intensive.

Procedure

  1. Open a fluorescent image/image stack.
  2.  Click Image>Modify image>Illumination correction. A check mark is placed next to the command and displays the Illumination Correction dialog box .
    • Type: Start with Gentle.
    • Light Removal: Start with 50%.
    • Target Channel: Apply to all channels or to an individual R,G,B channel.
  3. Click OK.
  4. Examine the effects of illumination correction. If you are not satisfied with the results:
    1. Close the image/image stack.
    2. Open the image/image stack again.
    3. Click Image>Image Illumination Correction again to select different settings.

 

In the absence of a background image, the program uses the image to estimate uneven illumination for background correction.