Image Stack Save saves the image stack with its original name and location, overwriting the original image stack.
Use Image Stack Save Asto save the modified stack without altering the original file.
To save the image only, without markers or tracing data, click Export the image file.
Example: neuron_ly_40x.tif
is saved as neuron_ly_40x_001
, neuron_ly_40x_002
, neuron_ly_40x_003.
Example: neuron_ly_40x.tif
is saved as neuron_ly_40x_R, neuron_ly_40x_G, neuron_ly_40x_B.
The Save image color option becomes available when you choose this option. This saves the color information with each separate channel or stack.
See Image Stack overview