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Open image stack

Use to open an image stack (a single file containing multiple images) or a stack of confocal images.

An image stack combines multiple images into a single image file or collection.

The images that make up a single stack can be stored in a single multi-image file or in individual files.

There are two formats for the multi-image file:

  • The PIC format is a BioRad proprietary format.
  • The TIF format is a non-proprietary format that also supports multiple images in a single file. Fluoview files are a type of TIF file.

Images stored in individual files can be loaded using the Image Stack Open command.

Some image stacks contain scaling information that the program uses to determine the distances between images.

If your stacks don't have scaling information, you can enter the distances to use manually via the Image Scaling dialog box.

  1. Click File>Open>Image Stack.
  2. In the Image Stack Open dialog box, select files and click Open. The program displays the Order of Files for Stack dialog box.
    If the files are not in the proper order, drag them in the list until the order is correct.
  3. Click OK. The Image Scaling dialog box is displayed.

    Since single image files do not contain Z spacing information, you need to enter a Z value manually.

  4. Click OK. The image stack is loaded.
  1. Click File>Open>Image Stack .
  2. In the Image Stack Open dialog box, select an image file, and click Open.
  3. Click OK. The program displays the Image Scaling dialog box.

    Scaling information is required if it is not already included in the image file.

  4. Click OK. The image stack is loaded.

 

If you load an image for which there is no matching lens, the program prompts you to create a new lens to match the image scaling. For more information, see Creating a lens.

 

See Image Stack overview, Serial section reconstruction from images