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Background correction

Slide scanning workflow—step 6

Depending on the type you selected in step 1 (brightfield or fluorescent), you'll see different setup options in the Background Correction panel.

The procedure for fluorescence requires Chroma background correction slides. These slides provide a uniform illumination and their intensity is even throughout the field of view. You can purchase those slides through Chroma or MBF Bioscience.

See detailed procedure in Background correction for brightfield modality.

Use Joy Track to navigate.

This option fills any white areas that are overexposed in the field of view with dark red pixels. If nothing appears when clip detection is enabled, raise the exposure or light level until the red pixels appear; keep the exposure between 10 and 100 ms.

Use the exposure slider from the Camera Settings window. Keep the exposure between 10 and 100 ms.

Adjust colors manually with the individual Red, Green, and Blue sliders to adjust the white balance.

Only applies to color cameras

A white balance adjustment is an alternative to a manual adjustment using the microscope's amplitude knob.

The image represented by this graph is color-balanced because the three colors have approximately the same functions on the graph. There are three methods to achieve white balance:

  • Select Area: Outline an area in the image that should be white.
  • Automatic: Explores the current image and balances the colors without specifying a white area.
  • Adjust colors manually with the individual Red, Green, and Blue sliders.

The background image is acquired then displayed in the main window.

See detailed procedure in Background correction for fluorescent modality.

This option fills any white areas that are overexposed in the field of view with dark red pixels. Make adjustments to the image until there are only a few red pixels visible (they may look like a light dusting). Your image should be very bright but not oversaturated.

The background image is acquired then displayed in the main window.