L-Cycloid Optical Fractionator formulas
Estimated length of lineal structure
- a/l: Area per unit cycloid length
: Number of intercepts- ssf: Section sampling fraction
- asf: Area sampling fraction
- t: Section cut thickness
- h: Height of counting frame
Area sampling fraction
- xCF,yCF: XY dimensions of counting frame
- xstep, ystep: Dimensions of grid
- area(Frame): Area of counting frame
- area(x,y step): Area of grid
References
Stocks, E. A., McArthur, J.C., Griffen, J.W., & Mouton, P.R. (1996). An unbiased method for estimation of total epidermal nerve fiber length. Journal of Neurocytology, 25 (1), 637–644.