1. Set up the subject

Procedure

Subject Information

Type your name, the subject name, and any notes that you want to include.

Sampling Parameters

Use an existing sampling configuration? Choose yes or no to use a previously saved sampling configuration or not.

If yes, click the browse icon.
In the Sampling Parameter Chooser window that opens, highlight the parameter set you wish to use and click OK.

Virtual section settings

Total height of image volume and Distance between image planes are automatically identified.
set Top (bottom) of ROI: Distance between the top (bottom) of the tissue and the top(bottom) of the virtual section.

To set the top or bottom of the region of interest (ROI), scroll the mouse wheel to focus through the section then click the SET TOP (BOTTOM) button.

Number of virtual sections: Number of desired virtual sections you will count for this subject. The appropriate number of virtual sections depends on your experimental goals, the density or sparsity of objects you will count, and the shape and size of your ROIs.
Virtual section thickness: Automatically calculated virtual section height (takes the "top" distance into account).
Disector height: Represents the height of the volume that you are going to count within the virtual section.