Cycloids for Lv

Purpose

Use the Cycloids for Lv probe to estimate the ratio of total line length to unit volume.

Stereo Investigator Cleared Tissue Edition software displays sets of short test curves (cycloids) in a grid pattern over the material to be examined.

You will place markers where the cycloids cross the projected lineal features of interest.

Requirements

  • Vertical slices of constant thickness with a random rotation around an identifiable vertical axis
  • Lineal features with a very small cross-sectional area.

Procedure

  1. Click Probes>Length>Cycloids for Lv to open the corresponding window.
  2. Enter Section Cut Thickness and Cycloid Width, then click OK. The program displays a test grid of cycloids and angles.

    The Cycloid Width is the vector distance from endpoint to endpoint of the cycloid line segment.

  3. Adjust the vertical axis.
    1. Right-click and select Define Vertical Axis.
    2. Drag the head of the arrow to align the white vertical axis with the vertical axis of the tissue.
    3. Right-click and select Accept.
  4. Click to mark wherever the cycloid curves cross the lineal features of interest.
    • Mark intersections in all focal planes of the tissue.
    • Mark the angles wherever they are located within the region of interest.
  5. When done marking all intersections, right-click and click Exit Cycloid.
  6. Results are displayed immediately in the Sampling Results window.

 

See Cycloids for Lv formulas