4. Set microscope to high magnification

Procedure

Select a higher objective that will be suitable for imaging the objects of interest (e.g. tubules, nerve fibers, small blood vessels, microvilli, etc.).
Select the corresponding lens from the Select High Mag Lens drop-down menu.

We suggest a high magnification lens with a high numerical aperture (generally oil immersion) to achieve thin focal planes necessary to properly discern intersection points.

Notes

The item to be counted is called a “profile;” it corresponds to the point where the fiber transects the sphere boundary.

If the fibers are thick, you must be able to identify the center point of the fiber and only count locations where the center point transects the sphere outline.