Trace your region(s) of interest

Physical Fractionator - step 3

1. Select the a section from the Working on drop-down menu.
2. Select a contour type from the Contour drop-down menu (use the same contour type for the same region of interest in subsequent sections).

   You can trace more than one contour if you are going to count different regions of a structure, different structures, or have more than one animal on a slide.

   If you are using different contours for different anatomical regions within the same animal, use a different contour type for each region and use the same contour types in subsequent regions. This will help you to distinguish between the 2 regions during analysis.

   Renaming contours after your area of interest

   1. Go to File>Preferences>Display Preferences>Contour.
   2. Click the contour type you would like to rename. This change will then be reflected in the workflow.
   Editing contours

   1. Click the Select icon.
   2. Click your contour; the program displays small white squares over contour points.
   3. Once the white squares are visible, right-click to access various editing tools.
   4. Right-click and select Exit the Selection Tool once you have finished editing.
3. Trace a contour.

   To change the tracing method, under Tracing Options, select a different method.

Tracing Options

Move the stage automatically when tracing outside dashed boundary (AutoMove): Check to keep your tracing aligned with the tissue (see AutoMove).