The options available at this step depend on the image source you selected at the previous step.
Use your microscope to make the image an appropriate size for tracing.
The objective should show a substantial portion of your region of interest in the field of view and is suitable for tracing a contour around your region of interest. If it is difficult to distinguish the anatomical borders of your region of interest at a low magnification, use a higher magnification for tracing.
Use the zoom buttons to size the image so that you can effectively trace regions of interest at the next step.