Set up the subject
Physical Fractionator - step 1
Don't forget to scroll down in your workflow to see the Image Source section.
Subject Information:
Type your name, the subject name, and any other information that you think will be helpful to someone who may work with this subject in the future.
Use Saved Sampling Parameters:
Choose whether or not to use a previously saved sampling configuration. If yes, click on the browse (…) icon. In the Sampling Parameter Chooser window, highlight the parameter set you wish to use and click OK.
Enter Section Information:
- Enter the distance between [section] pairs.
Example
If the cut section thickness (block advance) is 5µm and you are using a periodicity of 10, the distance between pairs is 50µm. For help determining the most suitable distance between pairs, Determining Sampling Precision
If you are using the Smooth Fractionator Method (Gundersen, 2002), keep note of the fraction you are using.
- Lookup Z position: The Lookup section is the second section in the pair.
- Reference Z position: The Reference section is the first section in the pair.
Example
- Cut thickness = 5µm
- Adjacent pairs
- You are starting with the very first cut pair.
Then the Reference Z Position is "0" and the Lookup Z Position is "5."
Image Source:
If you are working from previously acquired images of individual sections
- Select Image File to enable the browse buttons are active (indicated in green and yellow for the reference and lookup sections).
- Load up the appropriate Reference and Lookup images.
If you are using a live image:
- Select Live Video; the browse buttons will be disabled.