Set microscope to high magnification

Optical Fractionator - step 4

  1. Select a higher objective with a numerical aperture greater than 1.
  2. Select the corresponding lens from the Select High Mag Lens drop-down menu.

We suggest 60x, 63x or 100x oil objectives for counting. These objectives provide a thin depth of field required to count accurately, especially when cells are on top of one another.

 

Lower power objectives, such as 10x, 20x, or 40x air objectives do not have a thin enough depth of field to allow you to distinguish the top of one cell from another.