Image Illumination Correction

Use Image Illumination Correction when working with images already acquired.

Be aware that:
Illumination correction is designed for fluorescent images -- applying this command to other types of images is likely to yield poor results.
This command is CPU-intensive.

1. Open a fluorescent image/image stack.
2.  Click Image>Image Illumination Correction. Stereo Investigator places a check mark next to the command and displays the Illumination Correction dialog box .
   • Type: Start with Gentle.
   • Light Removal: Start with 50%.
   • Target Channel: Apply to all channels or to an individual R,G,B channel.
3. Click OK.
4. Examine the effects of illumination correction. If you are not satisfied with the results:
   1. Close the image/image stack.
   2. Open the image/image stack again.
   3. Click Image>Image Illumination Correction again to select different settings.

How it works

In the absence of a background image, Stereo Investigator uses the image to estimate uneven illumination for background correction.

 

Enable Illumination Correction for live images.

---

Stereo Investigator 11 | MBF Bioscience Support Center | Downloads