HDR Imaging high dynamic range
The HDR Imaging dialog box is used for both HDR Calibration and HDR Setup. 
Calibrate the camera (Acquisition>HDR Calibration) before defining the settings for the HDR acquisition with HDR Setup. 
HDR Calibration
- Use a specimen with a wide range of intensities (the specimen doesn't need to be representative of the specimens to be acquired).
- Exposure Range: Use exposures suitable for the observation of both underexposed and overexposed pixels.
- Lowest exposure value: Adjust the camera histogram so that the image is not saturated.
- Highest exposure value: Adjust the exposure so that you can see details in the darkest areas.
- Number of Exposures: 8 to 15 (greater than the number of exposures used for HDR Setup)
HDR Setup
- Exposure range:
- Lowest exposure value: Adjust the exposure until the maximum value on the camera histogram is at approximately 50% to avoid overexposed pixels.
- Highest exposure value: Adjust the exposure so that you can see detail in the dimmest area of the specimen.
- Number of exposures: 3 to 5 (increasing the number of exposures typically lead to better results, but the acquisition process takes longer)
See High Dynamic Range (HDR) Overview to learn more about HDR acquisitions
Stereo Investigator 11 | MBF Bioscience Support Center | Downloads