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Image Scaling (and Placement)

When you open an image, you might see one of these two windows: Image Scaling or Image Scaling and Placement.

Image Scaling can be used to override the default scaling (i.e., the scaling associated with the current lens) to apply the correct scaling information (i.e., settings at time of acquisition).

Image Scaling and Placement appears when Stereo Investigator "suspects" that the image placement should be re-calculated.

 

If you are loading an image from an external image source, use External Image Source Setup before loading the image.

X and Y Scaling Applied to Images

By default, Stereo Investigator applies the scaling settings associated with the current lens.

For some acquired images, you may need to override the default scaling to apply the correct scaling information (i.e., settings at time of acquisition).

This section allows you to:

ClosedSource Of Scaling
  • Check Override X and Y scaling to change the source.
  • If you select a lens other than the current lens, we recommend that you also check Recalculate the placement of the image assuming the image should be centered on the optical center under Image Placement.
  • If you select Specified Lens, then select a lens with parcentric correction enabled between the specified lens and the current lens, Stereo Investigator automatically uses the parcentric values used for the correction as Additional Offsets under Image Placement.
ClosedOverride X and Y scaling

Check to change the Source of Scaling.

Z Scaling applied to images

ClosedDistance between images

Use the focal distance or the physical distance.

ClosedFocal Distance

Image stacks collected with Stereo Investigator are collected using spacings that describe the focal plane separation.

ClosedPhysical Distance

Describes the physical movement of the microscope stage as images are collected.
If you select this option, you need to select the correction factor for the physical distance between the lens and the image. Stereo Investigator automatically enter this value for Air, Oil, and Water. If you select Other, you must manually enter the factor.

Image Placement (Image Scaling and Placement window only)

ClosedPlace Image in upper left hand corner of the main window

Select if you are only interested in viewing an image and you are not concerned with aligning with data file elements.

ClosedRecalculate the placement of the image assuming the image should be centered on the optical center

Select if you are loading an image from an external image source such and need that image to align with a live image, an acquired image from the camera, or a tracing made using the camera.

If this option is already selected, this is a good indication that:

  • The size of the image source does not match the size of the image being loaded

OR

  • Stereo Investigator has determined that the scaling is incorrect.
ClosedAdditional offsets (microns)

Even with the recalculation of the placement of the image, the field of view for the camera and the external source may not be exactly the same. As a result, the images from the camera and the external image source may not align perfectly.

Use additional offsets to refine the alignment -- this method is similar to performing a parcentric correction between two image sources.

  • If you select Specified Lens under Source of Scaling (see below), then select a lens with parcentric correction enabled between the specified lens and the current lens, Stereo Investigator automatically uses the parcentric values used for the correction as Additional Offsets.

 

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Stereo Investigator 11 | MBF Bioscience Support Center | Downloads